TIFA Antibody (N-term) [APR06179G]

TIFA Antibody (N-term) [APR06179G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TIFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-79 amino acids from the N-terminal region of human TIFA.

Cellular Localisation: Cytoplasm. Note=Colocalizes with lysosomal marker LAMP2 following homooligomerization and subsequent activation

Molecular Weight: 21445

Clone: Polyclonal

Gene Name: TIFA {ECO:0000303|PubMed:12566447, ECO:0000312|HGNC:HGNC:19075}

Gene ID: 92610

Function: Adapter molecule that plays a key role in the activation of proinflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed:12566447, PubMed:15492226, PubMed:26068852, PubMed:28877472, PubMed:28222186, PubMed:30111836) . Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (PubMed:15492226, PubMed:26068852) . TIFA-dependent innate immune response is triggered by ADP-D-glycero- beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram- negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of proinflammatory NF- kappa-B signaling (PubMed:30111836) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000 WB--1:1000

Synonyms: TRAF-interacting protein with FHA domain-containing protein A, Putative MAPK-activating protein PM14, Putative NF-kappa-B-activating protein 20, TRAF2-binding protein, TIFA, T2BP