Tim-3, Human Recombinant, (Fc-Tag Mouse) | P1358

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by administration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade

P1358 | Tim-3 Human Recombinant Fc-Tag Mouse DataSheet

Biomolecule/Target: Tim-3

Synonyms: TIM3; TIMD3; Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T Cell Immunoglobulin and Mucin Domain-containing Protein 3, HAVCR2

Alternates names: PWTSR, THSD2, Roof plate-specific spondin-3, RSPO3

Taglines: Mediate phagocytosis of apoptotic cells and inhibit aggressive Th1-mediated auto- and alloimmune responses.

NCBI Gene ID #: 84870

NCBI Gene Symbol: RSPO3

Gene Source: Human

Accession #: Q9BXY4

Recombinant: Yes

Source: CHO Cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

Biological activity: R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 37.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH