TIMP-1, human neutrophil granulocytes | 7787

The tissue inhibitor of metalloproteinases (TIMP-1) is a glycoprotein with a molecular mass of about 28-kDa. The secreted protein consists of 184 amino acids, six disuilfide bonds and two glycosylation sites containing N-linked oligosaccharides. TIMP-1 specifically inhibits the extracellular-matrix-egradingmetalloproteinases such as collagenase, Gelatinase and stromelysin. It has no activity on mammalian membrane metallo-proteinases, procollagen peptidase, or on bacterial metalloproteinases such as thermolysin

7787 | TIMP-1 human neutrophil granulocytes DataSheet

Biomolecule/Target: N/A

Synonyms: Tissue Inhibitor of Matrix Metalloproteinases – 1

Alternates names: Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)

Taglines: An inhibitor of MMPs

NCBI Gene ID #: 4049

NCBI Gene Symbol: TNFB

Gene Source: Human

Accession #: P01374

Recombinant: No

Source: Human neutrophil granulocytes (Buffy Coat)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of >2 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl; 1 M ZnCl; 0.05% Brij 35; 0,05% NaN

Reconstitution Instructions: Reconstitute in PBS to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long term storage, it is recommended to add a carrier protein (0.1% HAS or BSA).

Amino acid sequence: N/A