TIP1;1, TIP1;2 | tonoplast intrinistic protein1-1, 1-2 (gamma) | AS09 492

TIP1;1, TIP1;2 | tonoplast intrinistic protein1-1, 1-2 (gamma) | AS09 492 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. (2001) .

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: collection of antibodies to tonoplast proteins AS09 482 | Anti-AtTIP1;1 | gamma TIP, rabbit antibodiesAS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibodiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 1000 (WB)

Molecular weight: 25, 8 | 23 kDa

Confirmed Reactivity: Arabidopsis thaliana, Raphanus sativus

Predicted Reactivity: Brassica napus Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Background: TIPs belong to MIP/aquaporin protein family. Alternative names: gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Vacuolar membrane

Research Area: Plant Biology