TIP47 Antibody | 3881

TIP47 Antibody | 3881 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (86%)

Immunogen: Anti-TIP47 antibody (3881) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TIP47.
The immunogen is located within the last 50 amino acids of TIP47.

Research Area: Signal Transduction, Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-4 μg/mL; IHC-P: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 3: Cat. No.1204 - K562 Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 47kD
Observed: 47kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIP47 expression profile in human cell lines detected by two independent anti-TIP47 antibodies that recognize different epitopes, 3883 against N-terminus domain and 3881 against C-terminus domain.  TIP47 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Induced Expression Validation (Figure 6-8) : TIP47 expression detected by anit-TIP47 antibodies was increased in Atg7 liver KO mice (figure 6) , after starvation (figure 7) , or after oleic acid treatment (figure 8) .

Isoform: Human TIP47 has 4 isoforms, including isoform 1 (434aa, 47kD) , isoform 2 (251aa, 28kD) , isoform 3 (433aa, 47kD) , isoform 4 (422aa, 46kD) . Mouse TIP47 has one isoform (437aa, 47kD) and rat TIP47 also has one isoform (438aa, 47kD) . 3883 can detect all human isoforms, and can also detect mouse and rat TIP47.

Purification: TIP47 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TIP47 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.