TLR3 Knockout Immortalized Mouse Dendritic Cell Line | T3034

TLR3 Knockout Immortalized Mouse Dendritic Cell Line | T3034

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDCTlr3^-/- cell line is an immortalized splenic CD8α⁺ subset (CD11c^high,B220^-,DEC205⁺,CD24^high,CD11b^-) of conventional dendritic cells derived from the TLR3 knockout CD11c:SV40LgT transgenic mice .

Biosafety:

II

Organism:

CD11c:LgT-Tg x Tlr3(tm1.Flv) C57BL6 Mouse

Source Organ:

Spleen

Growth Properties:

Adherent

Morphology:

Small Aggregates

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

20,000 – 60,000 cells/cm².

Recommended split ratio: no greater than 1:3.

Markers:

CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is IMDM 1x) + Glutamax^TM Gibco Ref: 31980-030). To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum

Preservation:

1. Freeze Medium: Complete growth medium with 50% decomplemented fetal calf serum (PAN biotech; P40-37500) and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C