TLR4 Antibody | 3141

TLR4 Antibody | 3141 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (80%) , Horse: (73%) , Rat: (71%) , Pig: (73%)

Immunogen: TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.
The immunogen is located within amino acids 30 - 80 of TLR4.

Research Area: Innate Immunity

Tested Application: E, WB, ICC, IHC-P, IF

Application: TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 92 kDa
Observed: 94 kDa

Validation: N/A

Isoform: N/A

Purification: TLR4 Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TLR4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TLR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TLR4 Antibody: TOLL, CD284, TLR-4, ARMD10, Toll-like receptor 4, hToll

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TLR4 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.