TLR4 Antibody [TLR4/230] | 33-472

TLR4 Antibody [TLR4/230] | 33-472 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Monkey, Pig, Dog, Rat, Guinea Pig

Homology: N/A

Immunogen: Recombinant human protein was used as the immunogen for the TLR4 antibody.

Research Area: Innate Immunity

Tested Application: Func, Flow, IF, IHC-P

Application: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the TLR4 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: TLR4/230

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: TLR4 Antibody: TOLL, CD284, TLR-4, ARMD10, Toll-like receptor 4, hToll

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This mAb reacts with human Toll-like receptor 2 (TLR4) . It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.