TLR4 / CD284, Human Recombinant | P1417

TLR4 is a member of Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR4 receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

P1417 | TLR4 / CD284, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: ARMD10, CD284, TLR-4, TLR4, TOLL

Alternates names: ARMD10, CD284, TLR-4, TLR4, TOLL

Taglines: TLR family member which plays a fundamental role in pathogen recognition and activation of innate immunity

NCBI Gene ID #: 7099

NCBI Gene Symbol: TLR4

Gene Source: Human

Accession #: O00206

Recombinant: True

Source: Insect Cells (sf9)

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE.

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA. The concentration of human recombinant MD2 that produces 50% optimal binding response with immobilized human recombinant TLR4 at 2 ug/mL (100 uL/well) is approximately 0.5 ug/mL.

Biological activity:

Results:

Binding Capacity: Measured by its binding ability in a functional ELISA. The concentration of human recombinant MD2 that produces 50% optimal binding response with immobilized human recombinant TLR4 at 2 ug/mL (100 uL/well) is approximately 0.5 ug/mL.

Unit Definition:

Molecular Weight: 70.5 kDa (C terminal 6x His tag)

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 24-631

Handling: Centrifuge the vial prior to opening.

Usage: