TLR5 Antibody | 3649

TLR5 Antibody | 3649 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5.
The immunogen is located within the last 50 amino acids of TLR5.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IF

Application: TLR5 antibody can be used for detection of TLR5 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1218 – HT29 Cell Lysate

Positive Control 2: Cat. No. 17-008 - THP-1 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 94 kDa
Observed: 94 kDa

Validation: N/A

Isoform: N/A

Purification: TLR5 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TLR5 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TLR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TLR5 Antibody: TIL3, SLEB1, MELIOS, TIL3, Toll-like receptor 5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TLR5 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-α and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires'disease and demonstrates the importance of TLR5 in the innate immune response.