TLR9 Antibody | 3739

TLR9 Antibody | 3739 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.
The immunogen is located within amino acids 840 - 890 of TLR9.

Research Area: Signal Transduction, Innate Immunity

Tested Application: E, WB, ICC, IF

Application: TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of TLR9 are known to exist; this antibody will detect both isoforms. TLR9 antibody is predicted to not cross-react with other TLR proteins.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 104, 111 kDa
Observed: 97 kDa

Validation: N/A

Isoform: N/A

Purification: TLR9 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TLR9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TLR9 Antibody: CD289, Toll-like receptor 9

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.