TMM85 Antibody (N-term) [APR04003G]

TMM85 Antibody (N-term) [APR04003G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TMM85 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human TMM85.

Cellular Localisation: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Could also be a single-pass transmembrane protein with cytosolic N-terminus and lumenal C-terminus.

Molecular Weight: 20087

Clone: Polyclonal

Gene Name: EMC4

Gene ID: 51234

Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656) . Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231) . Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151) . It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231) . By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N- terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835) . By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable) .

Summary: Tissue Location: Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85, EMC4, TMEM85