TMPRSS2 (CT) Antibody | 9569

TMPRSS2 (CT) Antibody | 9569 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Monkey (100%) ; Gorilla (100%) ; Cat (92%) .

Immunogen: Anti-TMPRSS2 antibody (9569) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human TMPRSS2.
The immunogen is located within the last 50 amino acids of TMPRSS2.

Research Area: Infectious Disease, COVID-19

Tested Application: E, IF, WB

Application: WB: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1203 – Human Testis Lysate

Positive Control 2: Cat. No. 17-003 – A549 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 54kD
Observed: 54 kD

Validation: N/A

Isoform: Human TMPRSS2 has 2 isoforms, including isoform 1 (492aa, 54kD) and isoform 2 (529aa, 57.7kD) . Mouse TMPRSS2 has 1 isoform (490aa, 54kD) . Rat TMPRSS2 has one isoform (490aa, 53kD) . 9569 can detect TMPRSS2 in human, mouse and rat.

Purification: TMPRSS2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TMPRSS2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TMPRSS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TMPRSS2 Antibody: Transmembrane protease serine 2, Serine protease 10, PRSS10, Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain.

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TMPRSS2 Antibody: TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells. It facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. It proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV) , human metapneumovirus (HMPV) , human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV) . TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) , and it is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.