TNF alpha protein | 30R-AT045

TNF alpha protein | 30R-AT045 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Cytokines & Growth Factors

Short Description: Purified recombinant Human TNF alpha protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: Tumor Necrosis Factor-a Human Recombinant protein containing 158 amino acids.

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 98% pure

Methode of Purification: TNF alpha protein was purified by a proprietary chromatographic technique.

Source: N/A

Concentration: N/A

Form & Buffer: Lyophilized from 20mM PBS, pH 7.2, with 10mM NaCl. Reconstitute in water to a concentration of 0.1 - 1.0mg/ml.  Please Note:  Buffer may change with new Lots.

Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: User optimized

Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is

Usage Recommendations: N/A

Assay Information: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.