TNFSF8, human recombinant | 7331

TNFSF8 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. TNFSF8 was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Recombinant human TNFSF8 protein, fused to His-tag at N-terminus, was expressed in E.coli.

7331 | TNFSF8 human recombinant DataSheet

Biomolecule/Target: TNFSF8

Synonyms: tumor necrosis factor ligand superfamily member 8, CD153, CD30L, CD30LG

Alternates names: tumor necrosis factor ligand superfamily member 8, CD153, CD30L, CD30LG

Taglines: A ligand for TNFRSF8/CD30, which is a cell surface antigen

NCBI Gene ID #: 7132

NCBI Gene Symbol: TNR1A

Gene Source: Human

Accession #: AAA36756

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 microgram of protein

Activity (Specifications/test method): N/A

Biological activity: The biological activity of sTNF-RI was determined by its inhibitory effect of the TNF- mediated cytotoxicity in murine L-929 cells. The ED for the effect in the presence of 0.25 ng/ml of recombinant TNF-, is 0.05 mg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 22 kDa (195 aa, 63-234 aa + His Tag), confirmed by MALDI-TOF.

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 0.4 M Urea.

Reconstitution Instructions: Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A