TO3-3PEG-Biotin Fluorophore | G959

RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including:

• small size
• lack of toxicity
• plasma and nuclear membrane permeability
• short intracellular half-life
• the accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure
• TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach Aptamer systems (Kong et. al. 2021)