TOP1 polyclonal Antibody | BS91366

TOP1 polyclonal Antibody | BS91366 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Application: WB ICC/IF IHC FC

Application Range: WB:1:1,000-1:5,000 ICC:1:50-1:500 IHC:1:50-1:200 FC:1:50-1:100

Background: DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. The antitumor agent camptothecin (CPT) reversibly stabilizes the covalent enzyme-DNA intermediate by inhibiting DNA religation. When a replication fork collides with a DNA Top1 cleavage complex, the covalently bound enzyme must be removed from the DNA 3' end before recombination-dependent replication restart. The tyrosyl-DNA phosphodiesterase Tdp1 and the structure-specific endonuclease Rad1-Rad10 function as primary alternative pathways of Top1 repair in Saccharomyces cerevisiae. In the budding yeast S. cerevisiae, DNA topoisomerases I and II can functionally substitute for each other in removing positive and negative DNA supercoils.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: TOP1 polyclonal Antibody detects endogenous levels of TOP1 protein.

Molecular Weight: 91 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: TOP1, DNA topoisomerase 1, DNA topoisomerase I, NUP98 fusion gene, TOP 1, TOP I, TOP1, TOP1, TOPI, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, TopoisomeraseI, Type I DNA topoisomerase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: