TPA, Human Recombinant (Sf9) | P1325

Tissue plasminogen activator (PLAT or tPA) is a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 545 amino acids (24-562 a.a) and having a molecular mass of 61.3 kDa (Molecular size on SDS-PAGE will appear at approximately 50-70 kDa). tPA is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

P1325 | TPA Human Recombinant Sf9 DataSheet

Biomolecule/Target: TPA

Synonyms: Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT and tPA.

Alternates names: Stem Cell Growth Factor-alpha

Taglines: Plays a role in cell migration and tissue remodeling.

NCBI Gene ID #: 4254

NCBI Gene Symbol: SCF

Gene Source: Human

Accession #: P21583

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.

Biological activity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 33.9 kDa

Concentration: N/A

Appearance: Sterile Filtered colorless solution

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: GHGARGAERE WEGGWGGAQE EEREREALML KHLQEALGLP AGRGDENPAG TVEGKEDWEM EEDQGEEEEE EATPTPSSGP SPSPTPEDIV TYILGRLAGL DAGLHQLHVR LHALDTRVVE LTQGLRQLRN AAGDTRDAVQ ALQEAQGRAE REHGRLEGCL KGLRLGHKCF LLSRDFEAQA AAQARCTARG GSLAQPADRQ QMEALTRYLR AALAPYNWPV WLGVHDRRAE GLYLFENGQR VSFFAWHRSP RPELGAQPSA SPHPLSPDQP NGGTLENCVA QASDDGSWWD HDCQRRLYYV CEFPF