TPO, human recombinant | 4351

TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide, which contains the erythropoietin-like domain of the full length TPO protein.

4351 | TPO human recombinant DataSheet

Biomolecule/Target: TPO

Synonyms: TPO, human recombinant

Alternates names: Stem Cell Growth Factor-beta

Taglines: Stimulates the proliferation and maturation of megakaryocytes

NCBI Gene ID #: 3815

NCBI Gene Symbol: SCF

Gene Source: Human

Accession #: P10721

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is 7.0 ng/ml.

Biological activity: Determined by the dose dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is 7.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.25x PBS and 1mM DTT.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF