TPPP Antibody | 42-789

TPPP Antibody | 42-789 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: The immunogen for this antibody is: C-DPSKDRAAKRLSLES

Research Area: Other

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) , Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313 (20) :4091-106; PMID: 18028908) . Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificiy: N/A

Positive Control 1: Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate

Positive Control 2: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 3: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein

User Note: N/A

BACKGROUND: N/A