TRAIP Antibody (C-term) [APR05083G]

TRAIP Antibody (C-term) [APR05083G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TRAIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-356 amino acids from the C-terminal region of human TRAIP.

Cellular Localisation: Nucleus, nucleoplasm. Nucleus, nucleolus. Chromosome. Cytoplasm Cytoplasm, perinuclear region. Note=In the nucleus, found in close proximity to PCNA, suggesting localization at replication foci (PubMed:26595769) . Localizes to DNA damage sites in response to replication stress (PubMed:26781088, PubMed:26595769, PubMed:26711499) .

Molecular Weight: 53294

Clone: Polyclonal

Gene Name: TRAIP {ECO:0000303|PubMed:26595769, ECO:0000312|HGNC:HGNC:30764}

Gene ID: 10293

Function: E3 ubiquitin ligase required to protect genome stability in response to replication stress (PubMed:25335891, PubMed:26781088, PubMed:27462463, PubMed:26711499, PubMed:26595769, PubMed:31545170) . Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription (By similarity) . Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex (By similarity) . Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3 (By similarity) . If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway (By similarity) . Only catalyzes ubiquitination of MCM7 when forks converge (By similarity) . Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: promotes ubiquitination of DPCs, leading to their degradation by the proteasome (By similarity) . Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity (PubMed:24553286) . The function in translesion synthesis is however controversial (PubMed:26595769) . Acts as a regulator of the spindle assembly checkpoint (PubMed:25335891) . Also acts as a negative regulator of innate immune signaling by inhibiting activation of NF-kappa-B mediated by TNF (PubMed:22945920) . Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation (PubMed:22945920) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: E3 ubiquitin-protein ligase TRAIP, 632-, RING finger protein 206, TRAF-interacting protein, TRAIP, RNF206, TRIP