TREX2 Antibody | 4971

TREX2 Antibody | 4971 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: TREX2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TREX2.
The immunogen is located within the last 50 amino acids of TREX2.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: TREX2 antibody can be used for detection of TREX2 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This TREX2 antibody will not cross-react with the related protein TREX1.

Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 2: Cat. No. 10-201 - Human Liver Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: TREX2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TREX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TREX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TREX2 Antibody: Three prime repair exonuclease 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TREX2 Antibody: TREX2 is one of two major human 3' to 5' exonucleases which are required for checkpoint signaling after DNA damage. While the related protein TREX1 is required for ATR expression and mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis, less is known about TREX2. Like TREX1, TREX2 is ubiquitously expressed in all tissues examined. X-ray crystallography studies of TREX2-single strand DNA complexes revealed that TREX2 binds DNA as a dimer and may act to displace the second DNA strand, suggesting that TREX2 unzips and denatures double stranded DNA and feed the substrate DNA strand into the TREX2 active site. At least two isoforms of TREX2 are known to exist.