TRIM25 Antibody (Center) [APR04146G]

TRIM25 Antibody (Center) [APR04146G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TRIM25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human TRIM25.

Cellular Localisation: Cytoplasm. Cytoplasm, Stress granule. Nucleus {ECO:0000250|UniProtKB:Q61510}

Molecular Weight: 70973

Clone: Polyclonal

Gene Name: TRIM25

Gene ID: 7706

Function: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599) . Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1 (PubMed:17392790, PubMed:30193849, PubMed:33849980) . Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712) . Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849) . Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755) . Mediates estrogen action in various target organs (PubMed:22452784) . Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784) . Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity) .

Summary: Tissue Location: Expressed in breast tumors (at protein level) . Ubiquitous.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: E3 ubiquitin/ISG15 ligase TRIM25, 632n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147