TROL | Thylakoid rhodanese-like protein | AS19 4257

TROL | Thylakoid rhodanese-like protein | AS19 4257 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana TROL (thylakoid rhodanase-like protein) protein sequence, UniProt: Q9M158, TAIR: AT4G01050

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: antibodies to proteins involved in photosynthetic electron transfer

Recommended Dilutions: 1: 1000 - 1 : 3000 (WB)

Molecular weight: 55-60 kDa

Confirmed Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Pisum sativum

Predicted Reactivity: Brassica rapa, Eutrema salsugineum Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: TROL protein can be used as a chloroplast dual-localization marker: for the thylakoids (the major portion of the protein) and the chloroplastic inner envelope. Since the envelopes make a very small portion of the total membranes, the envelope form of TROL is detectable only when isolated envelopes are applied. For AgriseraECLBright and Agrisera matching secondary antibodies goat anti-rabbit HRP conjugated, dilution 1: 25 000 1h/RT incubation (AS09 602), anti-TROL antibodies can be used in dilution of 1:3000.Recommendation: 2 µg chlorophyll/well. With increased protein or chlorophyll load/well background bands may be detected. Check here.

Background: TROL (thylakoid rhodanase-like protein) is an integral membrane component associated to the photosynthetic apparatus of higher plants. TROL is involved in the final step of photosynthetic electron transport by binding a key energy-conversion enzyme ferredoxin: NADP+ oxidoreductase (FNR) . This interaction enables direct transfer of photosynthetic electrons from iron-sulphur protein ferredoxin at the stromal site of photosystem I to FNR, which then hands over electrons to NADP+. TROL is located in two distinct chloroplast compartments - in the inner envelope of chloroplasts, in its precursor form; and in the thylakoid membranes, where it is processed completely. Alternative names: Protein THYLAKOID RHODANESE-LIKE1, Sulfurtransferase 4, AtStr4

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Lyophilized antibody can be stored at -20°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G01050

Category: Electron transfer

Research Area: Plant Biology, Photosynthesis