TRPV4 Antibody (N-term) [APR13829G]

TRPV4 Antibody (N-term) [APR13829G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This TRPV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human TRPV4.

Cellular Localisation: Cell membrane. Apical cell membrane; Multi-pass membrane protein. Cell junction, adherens junction {ECO:0000250|UniProtKB:Q9EPK8}. Cell projection, cilium. Note=Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum [Isoform 5]: Cell membrane [Isoform 4]: Endoplasmic reticulum

Molecular Weight: 98281

Clone: Polyclonal

Gene Name: TRPV4

Gene ID: 59341

Function: Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040, PubMed:29899501) . Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18826956, PubMed:18695040, PubMed:25256292, PubMed:20037586, PubMed:21964574) . Increase of intracellular Ca (2+) potentiates currents. Channel activity seems to be regulated by a calmodulin- dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956) . Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity) . Acts as a regulator of intracellular Ca (2+) in synoviocytes (PubMed:19759329) . Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4- alpha-phorbol 12, 13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329) . Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040) . Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity) . Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity) . Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity) . Required for normal development and maintenance of bone and cartilage (PubMed:26249260) . In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (PubMed:29899501) .

Summary: Tissue Location: Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, TRPV4, VRL2, VROAC