Tubulin beta chain | AS10 681

Tubulin beta chain | AS10 681 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636 (At5g44340), beta-5 chain P29513 (At1g20010), beta-6 chain P29514 (At5g12250), beta-7 chain P29515 (At2g29550), beta-8 chain P29516 (At5g23860), beta-9 chain P29517 (At4g20890) .

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: immunofluorescence (IF), Western blot (WB)

Related Products: AS10 680 | Anti-tubulin alpha chain, rabbit antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: 1 : 1000 (IF), 1 : 500 (WB)

Molecular weight: 49 | 49 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa

Predicted Reactivity: Brassica napus, Glycine max, Hordeum vulgare, Micromonoas pusilla, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrium patens, Populus trichocarpa, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.

Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G62690

Category: Cytoskeleton

Research Area: Cell Biology