TWEAK Antibody | 3791

TWEAK Antibody | 3791 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Anti-TWEAK antibody (3791) was raised against recombinant human TWEAK protein.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-4 μg/mL; IHC: 5-10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 27 and 36 kD
Observed: 27 and 36 kD

Validation:

Recombinant Protein Test (Figure 2) : Anti-TWEAK antibodies (3791) detected human TWEAK recombinant protein at different concentrations.

Isoform: Human TWEAK has 2 isoforms, including isoform 1 (249aa, 27kD) and isoform 2 (330aa, 36kD) . Mouse TWEAK has one isoform (249aa, 27kD) and Rat TWEAK also has one isoform (249aa, 27kD) .

Purification: TWEAK Antibody is Protein A-purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: TWEAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TWEAK Antibody: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.