Tyrosine Hydroxylase (phospho Ser31) Antibody | XPS-1032

Tyrosine Hydroxylase (phospho Ser31) Antibody | XPS-1032 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse, Rat

Homology: N/A

Immunogen: TH (Ser31) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser31 of rat tyrosine hydroxylase (TH) .

Research Area: Neuroscience, Signal Transduction, Phospho-Specific

Tested Application: WB, IF, IHC

Application: Applications include Dot Blots (DB) , Immunoblotting (IB) , Immunofluorescence (IF) , Immunohistochemistry (IHC) and Western Blots (WB) . Mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.

Specificiy: Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser31 in PC-12 cells stimulated by Okadaic Acid.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 60

Validation: N/A

Isoform: N/A

Purification: Affinity Purified

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: N/A

Concentration: N/A

Storage Condition: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.