UBC polyclonal Antibody | BS70529

UBC polyclonal Antibody | BS70529 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC IF

Application Range: WB 1:1000 - 1:4000 IHC/IF 1:50 - 1:200

Background: Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1) . The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3) . Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4) . UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5) .

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: UBC polyclonal Antibody detects endogenous levels of UBC protein.

Molecular Weight: ~ 12, 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: UBC; HMG20; Ubiquitin;

Immunogen: A synthetic peptide of human UBC.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Phospholipase Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,Mitochondrial Control of Apoptosis,ErbB HER Signaling,