Ubiquitin Aldehyde, human recombinant | 4845

Ubiquitin-Aldehyde is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Aldehyde group. Ub-Aldehyde can be used for activity profiling experiments and DUB inhibitor specificity determination. It targets four of the five major DUB families: UCH, USP, OUT, and MJD, while leaving JAMM metalloproteases uninhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin-Aldehyde concentration of 1-5 M.

4845 | Ubiquitin Aldehyde human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Ubiquitin Aldehyde

Alternates names: Ubiquitin Aldehyde

Taglines: An inhibitor of deubiquitinylating enzymes (DUBs)

NCBI Gene ID #: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: Q96KJ0

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 97%

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): UCHL3 activity with Ubiquitin-AMC measured in the presence of 17.5 nM and 0.1 nM Ubiquitin Aldehyde.

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.2 kDa

Concentration: 350 uM, 3.1 mg/mL

Appearance: Liquid

Physical form description: In 50 mM NaOAc pH 5.0

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A