UCHL3 Mouse Recombinant | P1068

Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus, solitaris, and area postrema, and in working memory. Recombinant mouse Uchl3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1068 | UCHL3 Mouse Recombinant DataSheet

Biomolecule/Target: Uchl3

Synonyms: Ubiquitin carboxyl-terminal hydrolase isozyme L3, Uchl3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin thioesterase L3

Alternates names: TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand, TL2

Taglines: N/A

NCBI Gene ID #: 22035

NCBI Gene Symbol: TRAIL

Gene Source: Murine

Accession #: P50592

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Assay #1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay #2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml.

Biological activity: Assay #1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay #2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 20.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN