UGT8 Antibody | 42-832

UGT8 Antibody | 42-832 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: N/A

Homology: Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-NGKYKRNGHIK

Research Area: Other

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml. Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Specificiy: Reported variants represent identical protein: NP_001121646.1, NP_003351.2.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 1461 - Rat Heart Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, UDP-galactose ceramide galactosyltransferase, UDP-galactose-ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8

User Note: N/A

BACKGROUND: N/A