ULK3 Antibody | 7585

ULK3 Antibody | 7585 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (95%) , Mouse: (89%) , Chicken: (79%)

Immunogen: ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3.
The immunogen is located within amino acids 230 - 280 of ULK3.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 52 kDa
Observed: 52 kDa

Validation: N/A

Isoform: N/A

Purification: ULK3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ULK3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ULK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ULK3 Antibody: Unc-51-like kinase 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1) . Knockout of ULK genes results in a severe defect in the autophagy pathway (2) . ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3) . ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4) . Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5) .