ULK4 Antibody | 7587

ULK4 Antibody | 7587 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: ULK4 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ULK4.
The immunogen is located within amino acids 1120 - 1170 of ULK4.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 140 kDa
Observed: 165 kDa

Validation: N/A

Isoform: N/A

Purification: ULK4 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ULK4 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ULK4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ULK4 Antibody: FAM7C1, REC01035Unc-51-like kinase 4

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1) . Knockout of ULK genes results in a severe defect in the autophagy pathway (2) . ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3) . ULK4 has been implicated as a gene involved in the development of hydrocephalus (3) . This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4) .