UPF1 Antibody | 15-930

UPF1 Antibody | 15-930 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 270-370 of human UPF1 (NP_002902.2) .

Research Area: Apoptosis, Cell Cycle

Tested Application: WB, IF

Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: 293T

Positive Control 2: SH-SY5Y

Positive Control 3: K-562

Positive Control 4: HL-60

Positive Control 5: Mouse liver

Positive Control 6: N/A

Molecular Weight: Observed: 130kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: UPF1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, RENT1, pNORF1, smg-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.