UVRAG Antibody | 7947

UVRAG Antibody | 7947 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG.
The immunogen is located within amino acids 490 - 540 of UVRAG.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: UVRAG antibody is human and mouse reactive.

Positive Control 1: Cat. No. 1402 - Mouse Lung Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 77 kDa
Observed: 75 kDa

Validation: N/A

Isoform: N/A

Purification: UVRAG antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: UVRAG antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: UVRAG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: UVRAG Antibody: p63, DHTX, VPS38, UV radiation resistance-associated gene protein, p63

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1) . UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2) . UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3) . UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4, 5) .