V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG) | AS09 467

V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG) | AS09 467 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, O23654, At1g78900

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Ammonium sulfate purified, total IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: Collection of antibodies to other vacuolar membrane proteinsPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 2000 (WB)

Molecular weight: 68.8 | 70 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana tabacum

Predicted Reactivity: Chlamydomonas reinhardtii, Brassica napus, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Oryza sativa, Ostreococcus lucimarinus, Phaseolus aureus, Populus balsamifera, Physcomitrium patens, Solanum lycopersicon, Triticum aestivum, Zea mays Species of your interest not listed? Contact us

Not reactive in: Thermotoga neapolitana

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Background: V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H (+) -ATPase subunit A, V-ATPase 69 kDa subunit

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G78900

Category: Vacuolar membrane

Research Area: Plant Biology