V-ATPase, a1 | vacuolar H+-ATPase subunit a isoform 1 | AS14 2822

V-ATPase, a1 | vacuolar H+-ATPase subunit a isoform 1 | AS14 2822 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, UniProt: Q8RWZ7-1, TAIR: AT2G28520

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: collection of antibodies to other vacuolar membrane proteinsPlant protein extraction buffer

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 93 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Capsella rubella, Cucumis sativus, Erythranthe guttata, Glycine soja, Lupinus angustifolius, Morus notabilis, Phaseolus vulgaris, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Background: V-ATPase subunit VHA-a1 is a subunit of the membrane-integral Vo subunit. VHA-a1 target the V-ATPase enzyme to the trans-golgi network/earl endosome (TGN/EE) in Arabidopsis thaliana. This enzyme is involved in acidification process of various compartements of eucaryotic cells. The protein is coded by VHA-a1 gene AT2G28520. Alternative names: vacuolar H (+) -ATPase subunit a1, V-ATPase 93 kDa subunit.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G28520

Category: Vacuolar membrane

Research Area: Plant Biology