V-ATPase | Epsilon subunit of tonoplast H+ATPase | AS07 213

V-ATPase | Epsilon subunit of tonoplast H+ATPase | AS07 213 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1, TAIR: At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1) .

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Related Products: AS09 577 | Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase, goat antibodiesAS07 213P | V-ATPase | Epsilon subunit of tonoplast H+ATPase | Blocking peptide other antibodies to vacuolar membranemarker antibodies for plant cellular compartmentsrecommended secondary antibody

Recommended Dilutions: 1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Molecular weight: 26 | 31 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis, Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera

Predicted Reactivity: Brachypodium dystachyon, Capsella rubella, Citrus clementina, Citrus unshiu, Citrus limon, Eucalypsus grandis, Glyxine max, Glycine soja, Lotus japonicus, Phaseolus sp., Physcomitrium patens, Populus trichocarpa, Prunus persica, Ricinus communis, Riticum aestivum, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor Theobroma cacao, Vitis vinifera, Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, RatSpecies of your interest not listed? Contact us

Not reactive in: Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Additional Information: V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G11150

Category: Ion metabolism

Research Area: Metabolomics