V5 (clone V5, E10) | AS15 3027

V5 (clone V5, E10) | AS15 3027 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG1

Purity: Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format: Liquid

Tested Application: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

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Recommended Dilutions: 1 : 500-1 :2000 (IHC), 1 : 1000-1: 3000 (WB)

Molecular weight: N/A

Confirmed Reactivity: V5-tagged fusion proteins

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Antibody is purified on Protein A.For western blot incubate antibody for one hour at room temperature.

Background: V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: V5/VSV

Research Area: Tag Antibodies