Vaspin, human recombinant | 4915

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 415 amino acids and having a molecular mass of 47 kDa.

4915 | Vaspin human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Serpin A12 precursor, Visceral adipose-specific serpin, Visceral adipose tissue- derived serine protease inhibitor, Vaspin, OL-64, SERPINA12, Serine (or cysteine) proteinase inhibitor,clade A, antitrypsin, -1 antiproteinase,

Alternates names: TNF-related weak inducer of apoptosis receptor, TNFRSF12A, FGF-inducible 14, TWEAKR

Taglines: A newly identified adipokine

NCBI Gene ID #: 51330

NCBI Gene Symbol: TNFRSF12A

Gene Source: Human

Accession #: Q9NP84

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-3.0 g/ml.

Biological activity: Determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-3.0 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 5.6 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 50 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP