VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5 | AS07 212

VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5 | AS07 212 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5, TAIR: AT3G01280, VDAC2 UniProt F4K3R8-1, TAIR: AT5G67500, VDAC3 UniProt: Q9SMX3-1, TAIR: AT5G15090, VDAC4 UniProt: Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt: Q9M2W6-1, TAIR: AT3G49920

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Blue Native PAGE (2D BN/SDS-PAGE), Western blot (WB)

Related Products: AS07 212-ALP | Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1, ALP-conjugated (40 µg) AS07 212-HRP | Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1, HRP-conjugated (40 µg) AS04 054 | Anti-AOX1/2 rabbit antibody, marker of mitochondrial inner membraneAS06 203A | Anti-Idh rabbit antibody, marker of mitochondrial matrixcollection of antibodies to other mitochondrial proteins

Recommended Dilutions: 1 : 500 (IL), 1 : 5000, 2-30 µg protein/lane (WB)

Molecular weight: 29 kDa (for Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Brassica rapa subsp. rapa, Citrus sinensis, Fortunella margarita Swingle, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens, Zea mays

Predicted Reactivity: Arabidopsis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii, Glycine max, diatoms, Saccharomyces cerevisiae

Additional Information: Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Background: VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G01280, AT5G57490, AT5G15090, AT5G67500, AT3G49920

Category: Mitochondria | Respiration

Research Area: Plant Biology