VEGF Antibody [4B2-8] | XP-5740-M

VEGF Antibody [4B2-8] | XP-5740-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.

Research Area: Signal Transduction, Growth Factors, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL) , a concentration of <0.2 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100uL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL.
Western Blot:
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.

Clonality: Monoclonal

Clone: 4B2-8

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: Vascular Endothelial Growth Factor, VEGF, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A,

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A