VEGF165, murine recombinant | 4364

VEGF (Vascular Endothelial Growth Factor) is a homodimeric protein secreted by a variety of Vascularized tissues. VEGF stimulates endothelial cell growth, angiogenesis, and capillary permeability. Murine VEGF is a 39 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.

4364 | VEGF165 murine recombinant DataSheet

Biomolecule/Target: VEGF165

Synonyms: Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

Alternates names: Vascular Adhesion Protein-1, AOC3, SSAO, HPAO, Copper Amine Oxidase, Membrane Primary Amine Oxidase

Taglines: A signal protein that stimulates vasculogenesis and angiogenesis

NCBI Gene ID #: 8639

NCBI Gene Symbol: VAP1

Gene Source: Human

Accession #: Q16853

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >16 pmoles/min/µg of VAP-1.

Biological activity: Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >16 pmoles/min/µg of VAP-1.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~80.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: GRGGDGGEPS QLPHCPSVSP SAQPWTHPGQ SQLFADLSRE ELTAVMRFLT QRLGPGLVDA AQARPSDNCV FSVELQLPPK AAALAHLDRG SPPPAREALA IVFFGRQPQP NVSELVVGPL PHPSYMRDVT VERHGGPLPY HRRPVLFQEY LDIDQMIFNR ELPQASGLLH HCCFYKHRGR NLVTMTTAPR GLQSGDRATW FGLYYNISGA GFFLHHVGLE LLVNHKALDP ARWTIQKVFY QGRYYDSLAQ LEAQFEAGLV NVVLIPDNGT GGSWSLKSPV PPGPAPPLQF YPQGPRFSVQ GSRVASSLWT FSFGLGAFSG PRIFDVRFQG ERLVYEISLQ EALAIYGGNS PAAMTTRYVD GGFGMGKYTT PLTRGVDCPY LATYVDWHFL LESQAPKTIR DAFCVFEQNQ GLPLRRHHSD LYSHYFGGLA ETVLVVRSMS TLLNYDYVWD TVFHPSGAIE IRFYATGYIS SAFLFGATGK YGNQVSEHTL GTVHTHSAHF KVDLDVAGLE NWVWAEDMVF VPMAVPWSPE HQLQRLQVTR KLLEMEEQAA FLVGSATPRY LYLASNHSNK WGHPRGYRIQ MLSFAGEPLP QNSSMARGFS WERYQLAVTQ RKEEEPSSSS VFNQNDPWAP TVDFSDFINN ETIAGKDLVA WVTAGFLHIP HAEDIPNTVT VGNGVGFFLR PYNFFDEDPS FYSADSIYFR GDQDAGACEV NPLACLPQAA ACAPDLPAFS HGGFSHN