Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    Vimentin  | 
                                                
Conjugate:  | 
                                                    RPE  | 
                                                
Research Area:  | 
                                                    Neuroscience | Neurogenesis | Cell Signaling | Cytoskeleton | Intermediate Filaments | Vimentin | Neuroscience | Cell Markers | Neuron Markers | Neural Stem Cell Markers | Stem Cells | Lineage Markers | Endoderm | Cancer | Tumor Biomarkers | Neuroreg  | 
                                                
Alternative Name:  | 
                                                    CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    Synthesized peptide derived from Internal of human vimentin.  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, IHC, ELISA  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    IgG  | 
                                                
Species Reactivity:  | 
                                                    Human, Mouse, Rat  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:1000), IHC (1:100), ELISA (1:10000); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects ~53kDa, endogenous levels of total vimentin protein.  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:1000 dilution of SPC-1338 was sufficient for detection of Vimentin in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Cytoplasm  | 
                                                
Tissue Specificity:  | 
                                                    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.  | 
                                                
Accession Number:  | 
                                                    NP_003371.2  | 
                                                
Gene ID:  | 
                                                    7431  | 
                                                
Swiss-Prot:  | 
                                                    P08670  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |