VTI1b Antibody | 8289

VTI1b Antibody | 8289 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b.
The immunogen is located within amino acids 150 - 200 of VTI1b.

Research Area: Autophagy

Tested Application: E, WB, IHC-P

Application: VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist; this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 14, 19, 26 kDa
Observed: 27 kDa

Validation: N/A

Isoform: N/A

Purification: VTI1b antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: VTI1b antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: VTI1b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Vesicle transport through interaction with t-SNAREs 1B, VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1) . They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2, 3) . It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4) .