WAPL Antibody | 7799

WAPL Antibody | 7799 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL.
The immunogen is located within amino acids 30 - 80 of WAPL.

Research Area: Homeostasis, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 131 kDa
Observed: 140 kDa

Validation: N/A

Isoform: N/A

Purification: WAPL antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WAPL antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WAPL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: WAPL Antibody: FOE, WAPL, KIAA0261, FOE, Wings apart-like protein homolog, Friend of EBNA2 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: WAPL (wings apart-like) , also known as WAPAL or FOE, is a 1, 190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1) . It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1, 2, 4) . WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3, 4) . WAPL is a new regulator of the development and metastasis of cancerous tissue (4, 5) .