WIPI2 Antibody | 7103

WIPI2 Antibody | 7103 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Rat

Homology: N/A

Immunogen: Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2.
The immunogen is located within the first 50 amino acids of WIPI2.

Research Area: Autophagy

Tested Application: E, WB

Application: WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 50 kDa

Validation: N/A

Isoform: N/A

Purification: WIPI2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: WIPI2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: WIPI2 Antibody: Atg21, ATG18B, CGI-50, WIPI-2, WD repeat domain phosphoinositide-interacting protein 2, WIPI49-like protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.