WISP-1, Human Recombinant | P1281

WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a -catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor-C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.

P1281 | WISP-1 Human Recombinant DataSheet

Biomolecule/Target: WISP-1

Synonyms: WNT-1 inducible signaling pathway protein-1, CCN4, Wnt-1-induced secreted protein, WISP-1

Alternates names: T-4, Hematopoietic system regulatory peptide, Seraspenide, TMSB4X, TB4X, TMSB4

Taglines: Belongs to CCN family of cysteine rich regulatory proteins.

NCBI Gene ID #: 7114

NCBI Gene Symbol: TMSB4X

Gene Source: Human

Accession #: P62328

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Pretreatment of primary lung fibroblasts with recombinant Thymosin 4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.

Biological activity: Pretreatment of primary lung fibroblasts with recombinant Thymosin 4, using a concentration of 0.5 - 10 ug/ml, produces a protective effect against hydrogen peroxide induced cell death.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 5.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: RMSDKPDMAE IEKFDKSKLK KTETQEKNPL PSKETIEQEK QAGES