XBP-1 Antibody | 3685

XBP-1 Antibody | 3685 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (83%)

Immunogen: XBP-1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human XBP-1.
The immunogen is located within the last 50 amino acids of XBP-1.

Research Area: Signal Transduction, Chemokines & Cytokines

Tested Application: E, WB, ICC

Application: XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1216 - PC-3 Cell Lysate

Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3: Cat. No. 17-011 - Hep G2 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: XBP-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: XBP-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.